High Performance Liquid Chromatography (Hplc)

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What you'll learn
Understand and define basics of chromatography by learning stationary phase, mobile phase and analyte
Explain chromatography principles by understanding of affinity theory (solubility & adsorption)
Explain the types of chromatography based on bed type, separation mechanism, and physical states of phases
Understand instrumental design of HPLC system by explaining HPLC compartments
Define mission and importance of HPLC compartments such as solvent reservoir, solvent delivery system (pump), sampler unit & injector, column, dedector, monitor
Evaluate mission of HPLC techniques in analytical chemistry by explaining qualitative and quantitative analysis
Define types of HPLC such as Normal Phase (NP HPLC), Reversed Phase (RP HPLC), Ion Exchange (IEX) and Size Exclusion Chromatography (SEC)
Evaluate and understand descriptors of HPLC such as Retention Time, Void Volume, Retention Factor, Selectivity, Efficiency (Teorical Plate Number), Resolution

Requirements
Require mobile/tablet/laptop/personal computer with internet
To be student, graduate and/or chemistry/pharma professional

Description
High Performance Liquid Chromatography (HPLC) techniques are so popular and common in pharma industry, cosmetic industry, chemistry industry, food industry, polymer industry and environment laboratories.High performance liquid chromatography (HPLC) is the crucial and integral analytical tool applied in all stages of drug discovery, development, and commercialization in the pharmaceutical industry, especially. At the each step of drug development, drug substances, ingredients and drug products are controlled and monitored by using HPLC techniques.In this course, chromotography, chromatography principles, types of chromatography, instrumental design of HPLC, HPLC Analysis, Types of HPLC and Descriptors of HPLC have been discussed within all technical details.HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) COURSE SCOPE1 INTRODUCTION 2 INTRODUCTION TO HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) 3 CHROMATOGRAPHY 3_1 History of Chromatography 3_2 What is Chromatography? 3_3 What is Stationary Phase? 3_4 What is Mobile Phase? 3_5 What is Analyte? 4 CHROMATOGRAPHY PRINCIPLES 4_1 Chromatographic Seperation 4_2 Chromatography (Affinity & Interaction) 4_3 Chromatography (Solubility & Adsorption) 4_4 Chromatography (Solubility & Polarity) 4_5 Chromatography (Solubility & Hidrophilic / Hidrophobic) 4_6 Let's Categorize Molecules! 5 TYPES OF CHROMATOGRAPHY 5_1 Chromatography Classification Based on Bed (Column Chromatography, Paper Chromatogrphy, Thin Layer Chromatography) 5_2 Chromatography Classification Based on Separation Mechanism (Affinity Chromatography, Ion Exchange Chromatography, Gel Filtration Chromatography) 5_3 Chromatography Classification Based on Physical States of Phases (Gas Chromatography, Liquid Chromatography) 6 INSTRUMENTAL DESIGN OF HPLC SYSTEM 6_1 Instrumental Design of HPLC System_Simple Technical Design 6_2 Instrumental Design of HPLC System_Technical Design 6_3 Instrumental Design of HPLC System_Equipment Compartments 6_4 Instrumental Design of HPLC System_Solvent Reservoir 6_5 Instrumental Design of HPLC System_Solvent Delivery System (Pump) 6_6 Instrumental Design of HPLC System_Sampler Unit & Injector & Autosampler 6_7 Instrumental Design of HPLC System_Column 6_8 Instrumental Design of HPLC System_Dedector 6_9 Instrumental Design of HPLC System_PC Monitor 7 HPLC ANALYSIS 7_1 HPLC in Analytical Chemistry 7_2 Qualitative Analysis 7_3 Quantitative Analysis 7_4 Quantitative Analysis Example 8 TYPES OF HPLC 8_1 Types of HPLC 8_2 Normal Phase HPLC (NP HPLC) 8_3 Reversed Phase HPLC (RP HPLC) 8_4 Ion Exchange Chromatography (IEX) 8_5 Size Exclusion Chromatography (SEC) 9 DESCRIPTORS OF HPLC & ANALYTICAL METHOD DEVELOPMENT9_1 Retention Time 9_2 Void Volume (Dead Volume) 9_3 Retention Factor 9_4 Selectivity 9_5 Efficiency (Teorical Plate Number) 9_6 Selectivity & Efficiency 9_7 Resolution 10 CONCLUSION

Overview
Section 1: Introduction

Lecture 1 Introduction

Section 2: Introduction to High Performance Liquid Chromatography (HPLC)

Lecture 2 Introduction to High Performance Liquid Chromatography (HPLC)

Section 3: Chromatography

Lecture 3 Chromatography

Section 4: Chromatography Principles

Lecture 4 Chromatography Principles

Section 5: Types of Chromatography

Lecture 5 Types of Chromatography

Section 6: Instrumental Design of HPLC System

Lecture 6 Simple Technical Design

Lecture 7 Technical Design

Lecture 8 Equipment Compartments

Lecture 9 Solvent Reservoir

Lecture 10 Solvent Delivery System (Pump)

Lecture 11 Sampler Unit & Injector & Autosampler

Lecture 12 Column

Lecture 13 Dedector

Lecture 14 PC Monitor

Section 7: HPLC Analysis

Lecture 15 HPLC in Analytical Chemistry

Lecture 16 Qualitative Analysis

Lecture 17 Quantitative Analysis

Lecture 18 Quantitative Analysis Example

Section 8: Types of HPLC

Lecture 19 Types of HPLC

Lecture 20 Normal Phase HPLC (NP HPLC)

Lecture 21 Reversed Phase HPLC (RP HPLC)

Lecture 22 Ion Exchange Chromatography (IEX)

Lecture 23 Size Exclusion Chromatography (SEC)

Section 9: Descriptors of HPLC & Analytical Method Development

Lecture 24 Retention Time

Lecture 25 Void Volume (Dead Volume)

Lecture 26 Retention Factor

Lecture 27 Selectivity

Lecture 28 Efficiency (Teorical Plate Number)

Lecture 29 Selectivity & Efficiency

Lecture 30 Resolution

Section 10: Conclusion

Lecture 31 Conclusion

Anyone who is willing to learn High Performance Liquid Chromatography (HPLC),Anyone who wants to learn the chemistry of the High Performance Liquid Chromatography (HPLC),Anyone who wants to be qualified in analytical chemistry, instrumental analysis and pharmaceutical industry,Anyone who need to use HPLC techniques in analytical chemistry and/or pharmacy in the learning process,Anyone who desire to be master in HPLC analysis by using technical/chemical background,Anyone who wants to get certification related with High Performance Liquid Chromatography (HPLC)

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